pEGFP Kindlin2
(Plasmid #105305)

• Purpose: To study effect of Kindlin 2 on Cell/ECM adhesion
• Depositing Lab: Kenneth Yamada
• Publication: Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	105305	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pEGFP-NV
  (Search Vector Database)
• Backbone manufacturer: modified pEGFP-N1. GFP is in BamH1 and Not 1 sites
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kindlin2
• Alt name: FERMT2
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• GenBank ID: NM_006832
• Entrez Gene: FERMT2 (a.k.a. KIND2, MIG2, PLEKHC1, UNC112, UNC112B, mig-2)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: AATGTCGTAACAACTCCGCCCC

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry
• Article Citing this Plasmid:
  • 1 Reference

Depositor Comments

Original Kindlin 2 (FERMT2) cDNA was from OriGene, Cat no. SC320413.