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pET30b-7+-SrtA
(Plasmid #105602)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105602 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S.aureus Sortase A, 7+ mutant
  • Species
    S.aureus
  • Insert Size (bp)
    500
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30b-7+-SrtA was a gift from Stephanie Dougan (Addgene plasmid # 105602 ; http://n2t.net/addgene:105602 ; RRID:Addgene_105602)
  • For your References section:

    Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling. Jeong HJ, Abhiraman GC, Story CM, Ingram JR, Dougan SK. PLoS One. 2017 Dec 4;12(12):e0189068. doi: 10.1371/journal.pone.0189068. eCollection 2017. PONE-D-17-29293 [pii] PubMed 29200433