sgLenti
(Plasmid #105996)

• Purpose: (Empty Backbone) single sgRNA expression vector
• Depositing Lab: Mike McManus
• Publication: Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062.

Backbone

• Vector backbone: pBR322
• Vector type: Mammalian Expression, Lentiviral, CRISPR ; S.pyogenes sgRNA expression vector
• Promoter: U6
• Selectable markers: Puromycin ; mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the 'empty' sgRNA expression vector with a 1286 bp stuffer sequence. AarI digest cuts out the stuffer sequence and leaves sticky ends on the backbone. sgRNA inserts should be of the format 5'-TTGG(N20) for the top strand and 5'-AAAC-(N20) for the bottom strand for T4 cloning.