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Addgene

pHC01
(Plasmid #106900)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106900 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    ColE1 origin with Kan resistance marker
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 6977
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LasR
  • Alt name
    Biobrick BBa_C0179
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    720

Gene/Insert 2

  • Gene/Insert name
    Methyl Halide Transferase
  • Alt name
    MHT
  • Species
    Batis maritima (sequence was E. coli codon optimized)
  • Insert Size (bp)
    693

Gene/Insert 3

  • Gene/Insert name
    Ethylene Forming Enzyme
  • Alt name
    EFE
  • Species
    Pseudomonas syringae (sequence was E. coli codon optimized)
  • Insert Size (bp)
    1053

Gene/Insert 4

  • Gene/Insert name
    Red Fluorescent Protein
  • Alt name
    RFP
  • Species
    Synthetic
  • Insert Size (bp)
    678

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LasR contains V83A, G120D mutations and MHT contains an an A157V mutation compared to the NCBI reference sequences. Plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHC01 was a gift from Jonathan Silberg (Addgene plasmid # 106900 ; http://n2t.net/addgene:106900 ; RRID:Addgene_106900)
  • For your References section:

    Ratiometric gas reporting: a non-disruptive approach to monitor gene expression in soils. Cheng HY, Masiello CA, Del Valle I, Gao X, Bennett GN, Silberg JJ. ACS Synth Biol. 2018 Jan 24. doi: 10.1021/acssynbio.7b00405. 10.1021/acssynbio.7b00405 PubMed 29366321