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1354 pcDNA3 flag FKHR deltaDB
(Plasmid #10694)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    FKHR deltaDB
  • Alt name
    FOXO1A
  • Alt name
    FKHR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Mutation
    deletion of amino acids 208-220
  • Entrez Gene
    FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1354 pcDNA3 flag FKHR deltaDB was a gift from William Sellers (Addgene plasmid # 10694)