Addgene - 608 pSG5L HA RB del ex4 Plasmid Data

Plasmid 10730: 608 pSG5L HA RB del ex4

Gene/insert name:
RB del exon4
Alt name:
retinoblastoma
Alt name:
RB
Insert size:
2800
Species:
H. sapiens (human)
Entrez Gene:
RB1 (RP11-174I10.1, OSRC, RB, p105-Rb, pRb, pp110)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Mutation:
deletion of exon4
Vector backbone:
pSG5L
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4000
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Stable binding to E2F is not required for the retinoblastoma protein to activate transcription, promote differentiation, and suppress tumor cell growth. Sellers et al (Genes Dev. 1998 Jan 1. 12(1):95-106. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10730" in your Materials and Methods section.