977 pSG5L HA PTEN A4
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10753||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1200
MutationA4: S380A, T382A, T383A, S385A
Entrez GenePTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:977 pSG5L HA PTEN A4 was a gift from William Sellers (Addgene plasmid # 10753 ; http://n2t.net/addgene:10753 ; RRID:Addgene_10753)
For your References section:Phosphorylation of the PTEN tail regulates protein stability and function. Vazquez F, Ramaswamy S, Nakamura N, Sellers WR. Mol Cell Biol. 2000 Jul . 20(14):5010-8. 10.1128/MCB.20.14.5010-5018.2000 PubMed 10866658
Map uploaded by the depositor.