Addgene - PKCalpha.FLAG Plasmid Data

Plasmid 10805: PKCalpha.FLAG

Gene/insert name:
PKC alpha
Alt name:
PKC-A
Alt name:
PRKCA
Insert size:
2300
Species:
B. taurus (bovine)
GenBank ID:
NM_174435
Entrez Gene:
PRKCA (BOS_19579, PKRCA)
Fusion protein or tag:
FLAG
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Alex Toker
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Effect of overexpression of protein kinase C alpha on rat lacrimal gland protein secretion. Hodges et al (Adv Exp Med Biol. 2002 . 506(Pt A):237-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10805" in your Materials and Methods section.