pUCdeltaSS-26X
(Plasmid
#108218)
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PurposeYeast reporter gene with glucocorticoid response element (GRE) upstream of CYC1 promoter driving lacZ expression
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGRE-CYC1-lacZ
- Promoter CYC1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GRE is 5-AGTAGCTAGAACATCCTGTAC
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCdeltaSS-26X was a gift from Didier Picard & Keith Yamamoto (Addgene plasmid # 108218 ; http://n2t.net/addgene:108218 ; RRID:Addgene_108218) -
For your References section:
Mammalian glucocorticoid receptor derivatives enhance transcription in yeast. Schena M, Yamamoto KR. Science. 1988 Aug 19. 241(4868):965-7. 10.1126/science.3043665 PubMed 3043665
