prp8-G736E
(Plasmid #108450)

• Purpose: prp8-G736E in pRS313 backbone for expression in yeast
• Depositing Lab: Christine Guthrie
• Publication: Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2193-2199. doi: 10.1073/pnas.1819020116. Epub 2019 Jan 23.

Backbone

• Vector backbone: pRS313
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRP8
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 8000
• Mutation: changed G736 to E
• GenBank ID: Z24732.1
• Entrez Gene: PRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: ATG AGT GGA CTA CCG CCC C
• 3′ sequencing primer: TCA GCT AAA TAC ATC GAT TTG TTC GGC C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry