Addgene - pRSCupGFP Plasmid Data

Plasmid 1086: pRSCupGFP

Gene/insert name:
GFP
Insert size:
700
Species:
jellyfish
Vector backbone:
pRS316-CUP
(Search Vector Database)
Backbone manufacturer:
Thiele, 1988
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SacI
Site destroyed during cloning:
No
5' sequencing primer:
M13R List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

Additional Reference: Thiele, D.J. (1988). ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene. Mol. Cell. Biol. 8, 2745-2752.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Self-seeded fibers formed by Sup35, the protein determinant of [PSI+], a heritable prion-like factor of S. cerevisiae. Glover et al (Cell 1997 May 30;89(5):811-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1086" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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