PurposeExpresses aa28 to C-terminus of Flp recombinase fused to FKBP CID
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||108756||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Cre/Lox, Synthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameFlp recombinase fragment aa28 to C-terminus
- Promoter pCAG
/ Fusion Proteins
- FKBP (N terminal on insert)
- NLS (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBW2273_pCAG-PV1-FKBP-L1-FlpO-28C-NLS-BGHpA was a gift from Wilson Wong (Addgene plasmid # 108756 ; http://n2t.net/addgene:108756 ; RRID:Addgene_108756)
For your References section:High-performance chemical- and light-inducible recombinases in mammalian cells and mice. Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J, Wong WW. Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7. 10.1038/s41467-019-12800-7 PubMed 31649244