Addgene - siSlug3 Plasmid Data

Plasmid 10905: siSlug3

Gene/insert name:
siSlug
Alt name:
Slug siRNA
Alt name:
Slug
Insert size:
60
Species:
H. sapiens (human)
Entrez Gene:
SNAI2 (SLUG, SLUGH1, SNAIL2, WS2D)
Vector backbone:
pLKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7032
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Sequence: 5'-CAG ACC CAT TCT GAT GTA AAG-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Gupta et al (Nat Genet. 2005 Oct . 37(10):1047-54. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10905" in your Materials and Methods section.