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pGEM hsp60
(Plasmid #10922)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10922 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pGEM-5Zf+
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsp60
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Entrez Gene
    Hspd1 (a.k.a. 60kDa, CPN60, HSP-60, HSP-65, Hsp60)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM hsp60 was a gift from Bob Weinberg (Addgene plasmid # 10922)

  • For your References section:

    An interaction between p21ras and heat shock protein hsp60, a chaperonin. Ikawa S, Weinberg RA. Proc Natl Acad Sci U S A. 1992 Mar 15. 89(6):2012-6. 10.1073/pnas.89.6.2012 PubMed 1347942
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