pcDNA3.1 IGF1R-BirA(R118G)-HA
(Plasmid #109232)

• Purpose: This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).
• Depositing Lab: Victor Dzau
• Publication: Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002406. doi: 10.1074/jbc.RA118.002406.

Backbone

• Vector backbone: pcDNA3.1 MCS-BirA(R118G)-HA
• Backbone manufacturer: Kyle Roux, Addgene plasmid #36047
• Backbone size w/o insert (bp): 5333
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IGF1R
• Alt name: Insulin-like Growth Factor 1 Receptor
• Species: H. sapiens (human)
• Entrez Gene: IGF1R (a.k.a. CD221, IGFIR, IGFR, JTK13)
• Promoter: T7
• Tags / Fusion Proteins:
  • BirA(R118G) (highly promiscuous form) (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry