pSum-LacZ-gfp
(Plasmid
#109285)
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PurposeExpresses gfp in mycobacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSUM36
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Backbone manufacturerfrom PMID 9218713
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP
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MutationGFP modified for expression in prokaryotes; includes an optimized translational initiation region (from pGreenTIR)
- Promoter LacZalpha
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP, originally modified for expression in prokaryotes and including an optimized translational initiation region from the plasmid pGreenTIR, was cloned from pGreenTIR into the HindIII site of the pSUM36 vector to generate pSum-LacZ-gfp.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSum-LacZ-gfp was a gift from Nicolai van Oers (Addgene plasmid # 109285 ; http://n2t.net/addgene:109285 ; RRID:Addgene_109285) -
For your References section:
Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages. Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Ainsa JA, Hansen EJ, Gumbo T, van Oers NS. Appl Environ Microbiol. 2012 Oct;78(19):6829-37. doi: 10.1128/AEM.01674-12. Epub 2012 Jul 20. 10.1128/AEM.01674-12 PubMed 22820329
