Addgene - pcDNA3.1hisA cdc25c C330S Plasmid Data

Plasmid 10966: pcDNA3.1hisA cdc25c C330S

Gene/insert name:
cdc25c C330S
Alt name:
cdc25c
Insert size:
1450
Species:
H. sapiens (human)
Entrez Gene:
CDC25C (CDC25, PPP1R60)
Fusion protein or tag:
6xHis
Terminal:
N terminal on backbone
Mutation:
C330S. Cysteine to Serine substitution at aa330.
Vector backbone:
pcDNA3.1 His A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGHrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
cdc25C was a gift from Dr. Helen Piwnica-Worms
Sequence: View sequences (1)
Principal Investigator:
Toren Finkel
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Redox regulation of Cdc25C. Savitsky et al (J Biol Chem. 2002 Jun 7. 277(23):20535-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10966" in your Materials and Methods section.