Addgene - pYES2.1-TRP1URA3 Plasmid Data

Plasmid 11008: pYES2.1-TRP1URA3

Gene/insert name:
TRP1URA3 fusion
Insert size:
1494
Species:
S. cerevisiae (budding yeast)
Fusion protein or tag:
his6
Terminal:
C terminal on backbone
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Mutation:
ORF-TRP1 fused to ORF-URA without stop codon
Vector backbone:
pYES2.1-TRP1
(Search Vector Database)
Vector type:
Bacterial Expression, Yeast Expression
Backbone size w/o insert (bp):
6558
Cloning site 5':
BstEII
Site destroyed during cloning:
No
Cloning site 3':
BstEII
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (3)
Principal Investigator:
Francisco Malagon
Terms and Licenses:
MTA

Comments:

Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11008" in your Materials and Methods section.