Addgene - EGFR D837A Plasmid Data

Plasmid 11014: EGFR D837A

Gene/insert name:
EGFR D837A
Alt name:
epidermal growth factor receptor
Alt name:
ERBB
Insert size:
3639
Species:
H. sapiens (human)
Entrez Gene:
EGFR (ERBB, ERBB1, HER1, PIG61, mENA)
Mutation:
D837A
Vector backbone:
pBABE puro
(Search Vector Database)
Backbone manufacturer:
available at Addgene
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
SnaBI
Site destroyed during cloning:
Yes
Cloning site 3':
SnaBI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Matthew Meyerson
Terms and Licenses:
MTA

Comments:

Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-GGTGCACCGCGCCCTGGCAGCCA-3' and 5'-TGGCTGCCAGGGCGCGGTGCACC-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. Greulich et al (PLoS Med. 2005 Oct 4. 2(11):e313. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11014" in your Materials and Methods section.