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Plasmid 11014: EGFR D837A
  • Retroviral construct for expressing human EGFR with D837A mutation in human cells

  • EGFR D837A

  • epidermal growth factor receptor

  • ERBB

  • 3639

  • H. sapiens (human)

  • EGFR (ERBB, ERBB1, HER1, PIG61, mENA)

  • D837A

  • pBABE puro
    (Search Vector Database)

  • available at Addgene

  • Mammalian Expression, Retroviral

  • 5169

  • SnaBI

  • Yes

  • SnaBI

  • Yes

  • pBABE5' List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (2)
  • Matthew Meyerson

  • UBMTA

Comments: 

Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-GGTGCACCGCGCCCTGGCAGCCA-3' and 5'-TGGCTGCCAGGGCGCGGTGCACC-3'

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. Greulich et al (PLoS Med. 2005 Oct 4. 2(11):e313. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11014" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only