EGFR D837A
(Plasmid #11014)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    available at Addgene
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    EGFR D837A
  • Alt name
    epidermal growth factor receptor
  • Alt name
    ERBB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3639
  • Mutation
    D837A
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, HER1, NISBD2, PIG61, mENA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-GGTGCACCGCGCCCTGGCAGCCA-3' and 5'-TGGCTGCCAGGGCGCGGTGCACC-3'

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFR D837A was a gift from Matthew Meyerson (Addgene plasmid # 11014)
  • For your References section:

    Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M. PLoS Med. 2005 Oct 4. 2(11):e313. 10.1371/journal.pmed.0020313 PubMed 16187797