pBABE.zeo/hKGA.S286A
(Plasmid #110357)

• Purpose: Mammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tag
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Mammalian retroviral expression plasmids based on pBABE family (unpublished)

Backbone

• Vector backbone: pBABE-zeo (pBABE-bleo)
• Backbone manufacturer: #1766
• Backbone size w/o insert (bp): 4902
• Total vector size (bp): 6990
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KGA glutaminase
• Alt name: GLS (KGA Isoform)
• Species: H. sapiens (human)
• Insert Size (bp): 2007
• Mutation: S286A
• GenBank ID: 2744 NM_014905
• Entrez Gene: GLS (a.k.a. AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, KGA)
• Promoter: gag
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5' 5'-CTTTATCCAGCCCTCAC-3'

Resource Information

• Supplemental Documents:
  • pD175-pBABE.zeocin_hKGA.S286A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.