p068 pCS mTRPS1 (M)
(Plasmid #11040)

• Depositing Lab: Ramesh Shivdasani
• Publication: Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Backbone

• Vector backbone: pCS105
• Backbone size w/o insert (bp): 4133
• Vector type: xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRPS1 mut
• Alt name: TRPS1
• Species: M. musculus (mouse)
• Insert Size (bp): 4300
• Mutation: Two Cys residues at positions 917 and 920 in the GATA-type zinc finger were mutated to yield the sequence VGNAGG instead of VCNACG.
• Entrez Gene: Trps1 (a.k.a. AI115454, AI447310, D15Ertd586e)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3 or M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full coding + UTR of mTRPS1. Can be expressed in cos cell, xenopus embryos, and in vitro translation.