UPATrap-VC-ORF2
(Plasmid
#110653)
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Purposetransposon containing BiFC VC cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110653 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUPATrap-TMat-08
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Backbone manufacturerGenbank AB673353
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVenus C-terminus cassette
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UPATrap-VC-ORF2 was a gift from Chris Lord (Addgene plasmid # 110653 ; http://n2t.net/addgene:110653 ; RRID:Addgene_110653) -
For your References section:
Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, Ashworth A, Lord CJ. Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4. 10.1038/s41467-018-04466-4 PubMed 29789535