Addgene - pET-15b EFN WT Plasmid Data

Plasmid 11078: pET-15b EFN WT

Gene/insert name:
EFN
Alt name:
edema factor N
Species:
Bacillus anthracis
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Mutation:
N terminus of EF
Vector backbone:
pET-15b
(Search Vector Database)
Backbone manufacturer:
EMD Biosciences
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5700
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Principal Investigator:
John Collier
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11078" in your Materials and Methods section.