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Plasmid 11079: pET-22b PA WT
Comments: 

NdeI site at start of mature protein. Stop codon, out of frame His tag on vector, then another stop codon. There are 10 extra amino acids at the 5' end of the coding sequence corresponding to a second leader sequence. E285Q is the correct sequence as determined by crystallography and different from Genbank.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11079" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only