pET-22b PA WT
Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5500
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameprotective antigen
Insert Size (bp)2200
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
NdeI site at start of mature protein. Stop codon, out of frame His tag on vector, then another stop codon. There are 10 extra amino acids at the 5' end of the coding sequence corresponding to a second leader sequence. E285Q is the correct sequence as determined by crystallography and different from Genbank.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-22b PA WT was a gift from John Collier (Addgene plasmid # 11079)
Map generated by Addgene from full sequence supplied by depositor.