pET-22b PA WT
(Plasmid #11079)

Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
  • Alt name
    protective antigen
  • Species
    Bacillus anthracis
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

NdeI site at start of mature protein. Stop codon, out of frame His tag on vector, then another stop codon. There are 10 extra amino acids at the 5' end of the coding sequence corresponding to a second leader sequence. E285Q is the correct sequence as determined by crystallography and different from Genbank.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-22b PA WT was a gift from John Collier (Addgene plasmid # 11079)