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pbabe-hTERT+p53DD
(Plasmid #11128)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11128 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pbabe
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hTERT, p53 dominant negative
  • Alt name
    hTERT (human)
  • Alt name
    p53DD (mouse)
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    4600
  • Mutation
    p53 dominant negative (aa1-14; 303-390)
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1(hTERT) HindIII (p53DD) (not destroyed)
  • 3′ cloning site SalI(hTERT) Cla I(p53DD) (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid was generated by cloning FLAG-hTERT cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site p53DD cDNA in which HindIII/ClaI sites were again added by PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pbabe-hTERT+p53DD was a gift from Christopher Counter (Addgene plasmid # 11128 ; http://n2t.net/addgene:11128 ; RRID:Addgene_11128)
  • For your References section:

    A network of genetic events sufficient to convert normal human cells to a tumorigenic state. Kendall SD, Linardic CM, Adam SJ, Counter CM. Cancer Res. 2005 Nov 1. 65(21):9824-8. 10.1158/0008-5472.CAN-05-1543 PubMed 16267004