Prp39-pET21b
(Plasmid
#111286)
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PurposePrp39 expression plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET21b
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5442
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePRP39
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Alt nameYML046W
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- his6 (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Prp39-pET21b was a gift from Christine Guthrie (Addgene plasmid # 111286 ; http://n2t.net/addgene:111286 ; RRID:Addgene_111286) -
For your References section:
An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8. Price AM, Gornemann J, Guthrie C, Brow DA. RNA. 2014 Jan;20(1):46-60. doi: 10.1261/rna.041970.113. Epub 2013 Nov 14. 10.1261/rna.041970.113 PubMed 24231520
