yCp50-PRP5
(Plasmid
#111303)
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PurposePrp5 in yCp50 plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneyCP50
- Backbone size w/o insert (bp) 7950
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePRP5
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Alt nameYBR237W
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Alt nameRNA5
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SpeciesS. cerevisiae (budding yeast)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full plasmid sequence contains several ambiguous bases in the backbone- Unsure if these regions will affect function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
yCp50-PRP5 was a gift from Christine Guthrie (Addgene plasmid # 111303 ; http://n2t.net/addgene:111303 ; RRID:Addgene_111303) -
For your References section:
Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Noble SM, Guthrie C. Genetics. 1996 May;143(1):67-80. PubMed 8722763
