pJU225 Prp8-TAP
(Plasmid #111305)

• Purpose: Prp8-TAP
• Depositing Lab: Christine Guthrie
• Publication: Umen et al Genetics. 1996 Jun;143(2):723-39.

Backbone

• Vector backbone: pRS424
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PRP8
• Alt name: RNA8
• Alt name: SLT21
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: PRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid pJU225 (PRP8 TRPl2p) was constructed from
a previously described plasmid JDYl3 (GAL::PRP8) (BROWN
and BEGGS 1992). The galactose driven promoter in this plasmid
was removed by cutting with NheI and XhoI. A wild-type
copy of the promoter was amplified by PCR under nonmutagenic
conditions and used to replace the galactose driven
promoter. The entire PRP8 gene was then excised using XhoI
and NotI and ligated to plasmid pRS424 (TRPl 2p) also cut
with XhoI and NotI.