pRS303 prp8-1
(Plasmid
#111306)
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Purposeprp8-1
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS303
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePRP8
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Alt nameSLT21
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Alt nameRNA8
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SpeciesS. cerevisiae (budding yeast)
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Mutationprp8-1 ( G1801D )
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Entrez GenePRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note PRP8 has a G1801D mutation- unsure if this affects protein function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS303 prp8-1 was a gift from Christine Guthrie (Addgene plasmid # 111306 ; http://n2t.net/addgene:111306 ; RRID:Addgene_111306) -
For your References section:
Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3' splice site selection. Umen JG, Guthrie C. Genetics. 1996 Jun;143(2):723-39. PubMed 8725222
