PurposeAn AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||111584||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4377
- Total vector size (bp) 6195
Growth in Bacteria
Growth Strain(s)NEB Stable
Insert Size (bp)1818
- Promoter tetO
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer None
- 3′ sequencing primer None (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP was a gift from Bong-Kiun Kaang (Addgene plasmid # 111584 ; http://n2t.net/addgene:111584 ; RRID:Addgene_111584)
For your References section:Interregional synaptic maps among engram cells underlie memory formation. Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, Lim CS, Kaang BK. Science. 2018 Apr 27;360(6387):430-435. doi: 10.1126/science.aas9204. 10.1126/science.aas9204 PubMed 29700265