piHGpd/A587T
(Plasmid #1116)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRS303
  • Backbone manufacturer
    ATCC (#77189)
  • Backbone size w/o insert (bp) 4453
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

  • Depositor Sequences
    None.
  • Addgene Sequences

    None. Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    HSP82
  • Alt name
    Hsp90
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3800
  • Mutation
    HSP82 with alanine 587 changed to threonine; regulated by the strong constitutive promoter GPD
  • GenBank ID
    K01387
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piHGpd/A587T was a gift from Susan Lindquist (Addgene plasmid # 1116)
  • For your References section:

    Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp90 loss-of-function mutation. Nathan DF, Vos MH, Lindquist S. Proc Natl Acad Sci U S A 1999 Feb 16;96(4):1409-14. 10.1073/pnas.96.4.1409 PubMed 9990037