Addgene - piHGpd/A587T Plasmid Data

Plasmid 1116: piHGpd/A587T

Gene/insert name:
HSP82
Alt name:
Hsp90
Insert size:
3800
Species:
S. cerevisiae (budding yeast)
GenBank ID:
K01387
Entrez Gene:
HSP82 (YPL240C, HSP90)
Mutation:
HSP82 with alanine 587 changed to threonine; regulated by the strong constitutive promoter GPD
Vector backbone:
pRS303
(Search Vector Database)
Backbone manufacturer:
ATCC (#77189)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
4453
Cloning site 5':
ClaI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp90 loss-of-function mutation. Nathan et al (Proc Natl Acad Sci U S A 1999 Feb 16;96(4):1409-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1116" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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