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Plasmid 1116: piHGpd/A587T
  • HSP82

  • Hsp90

  • 3800

  • S. cerevisiae (budding yeast)

  • K01387

  • HSP82 (YPL240C, HSP90)

  • HSP82 with alanine 587 changed to threonine; regulated by the strong constitutive promoter GPD

  • pRS303
    (Search Vector Database)

  • ATCC (#77189)

  • Yeast Expression

  • 4453

  • ClaI

  • No

  • NotI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Susan Lindquist

  • UBMTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp90 loss-of-function mutation. Nathan et al (Proc Natl Acad Sci U S A 1999 Feb 16;96(4):1409-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1116" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with