pSe-Rp1
(Plasmid
#111644)
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Purpose(Empty Backbone) For construction of spacer and repeat arrays for Sulfolobus islandicus REY154A
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonenot applicable
- Backbone size (bp) 8434
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Vector typearchaeal expression vector
- Promoter araS
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsOther growth strain: Sulfolobus islandicus Rey15A
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer 5'-TCTCTCTCTAGAGCCGTGC-3'
- 3′ sequencing primer 5'-ATGTTAAACTACGCCAGTAGG-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSe-Rp1 was a gift from Qunxin She (Addgene plasmid # 111644 ; http://n2t.net/addgene:111644 ; RRID:Addgene_111644) -
For your References section:
An archaeal CRISPR type III-B system exhibiting distinctive RNA targeting features and mediating dual RNA and DNA interference. Peng W, Feng M, Feng X, Liang YX, She Q. Nucleic Acids Res. 2015 Jan;43(1):406-17. doi: 10.1093/nar/gku1302. Epub 2014 Dec 10. 10.1093/nar/gku1302 PubMed 25505143
