Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA cyclin D1 HA T286A
(Plasmid #11182)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11182 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3?
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D1 T286A
  • Alt name
    cyclin D1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1120
  • Mutation
    T286A. Mutant that cannot be degraded via the ubiquitin pathway.
  • GenBank ID
    BC025302
  • Entrez Gene
    CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Constructed using site-directed mutagenesis on the parent plasmid, pcDNA cyclinD1 HA, using the primers 5'-acctggcttgcgcacccaccgacg-3' and 5'-cgtcggtgggtgcgcaagccaggt-3'.

Note: The author's map and author's sequence are not fully correct. EcoRI and XhoI are not unique sites in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA cyclin D1 HA T286A was a gift from Bruce Zetter (Addgene plasmid # 11182 ; http://n2t.net/addgene:11182 ; RRID:Addgene_11182)
  • For your References section:

    Antizyme targets cyclin D1 for degradation. A novel mechanism for cell growth repression. Newman RM, Mobascher A, Mangold U, Koike C, Diah S, Schmidt M, Finley D, Zetter BR. J Biol Chem. 2004 Oct 1. 279(40):41504-11. 10.1074/jbc.M407349200 PubMed 15277517