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pBABE-bleo p85alpha M/D
(Plasmid #11208)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11208 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBabe-bleo
  • Backbone size w/o insert (bp) 4888
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p85alpha
  • Species
    H. sapiens (human)
  • Mutation
    Wildtype: Met-326, Asp-330
  • Entrez Gene
    PIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-bleo p85alpha M/D was a gift from Ronald Kahn (Addgene plasmid # 11208)

  • For your References section:

    Characterization of the Met326Ile variant of phosphatidylinositol 3-kinase p85alpha. Almind K, Delahaye L, Hansen T, Van Obberghen E, Pedersen O, Kahn CR. Proc Natl Acad Sci U S A. 2002 Feb 19. 99(4):2124-8. 10.1073/pnas.042688799 PubMed 11842213
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