pGreenFire1_E2F1RE
(Plasmid
#112248)
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Purposeexpression of GFP and Luciferase under the control of E2F1 responsive element
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Depositing Lab
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Depositing OrganizationImperial College London
Located in United Kingdom of Great Britain and Northern Ireland -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGreenFire1
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE2F1RE (responsive element)
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Tags
/ Fusion Proteins
- copGFP (C terminal on backbone)
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGreenFire1_E2F1RE was a gift from Simone Di Giovanni (Addgene plasmid # 112248 ; http://n2t.net/addgene:112248 ; RRID:Addgene_112248)