pQE30-mBFP
(Plasmid #112682)

• Purpose: Expression of NADPH-dependent blue fluorescent protein
• Depositing Lab: Geun-Joong Kim
• Publication: Hwang et al Biochem Biophys Res Commun. 2012 Mar 23;419(4):676-81. doi: 10.1016/j.bbrc.2012.02.075. Epub 2012 Feb 20.

Backbone

• Vector backbone: pQE30
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3461
• Total vector size (bp): 4174
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: This mBFP is highly expressed as functional form without adding IPTG.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: His-mBFP
• Alt name: metagenomic derived blue-fluorescent protein
• Species: metagenome-derived gene
• Insert Size (bp): 786
• GenBank ID: GU460154.1
• Promoter: T5
• Tag / Fusion Protein:
  • His tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SphI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pQE-F
• 3′ sequencing primer: pQE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry