PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112879||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Entrez GeneSGR_RS01140 (a.k.a. SGR_RS01140, SGR_249)
- Promoter Pbla
/ Fusion Protein
- 6xHis (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CATGAGACAATAACCCTG
- 3′ sequencing primer CCAACTCAGCTTCCTTTC (Common Sequencing Primers)
Please note that some discrepancies were found between Addgene's quality control and the depositor's insert sequence. The depositor noted that these discrepancies do NOT affect plasmid function. JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGDP3 aph(3'')-Ia was a gift from Gerard Wright (Addgene plasmid # 112879 ; http://n2t.net/addgene:112879 ; RRID:Addgene_112879)
For your References section:A Common Platform for Antibiotic Dereplication and Adjuvant Discovery. Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, Wright GD. Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22. 10.1016/j.chembiol.2016.11.011 PubMed 28017602