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pTP813
(Plasmid #113308)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113308 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mre11
  • Species
    H. sapiens (human)
  • Entrez Gene
    MRE11 (a.k.a. ATLD, HNGS1, MRE11A, MRE11B)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP813 was a gift from Tanya Paull (Addgene plasmid # 113308 ; http://n2t.net/addgene:113308 ; RRID:Addgene_113308)
  • For your References section:

    Rad50 adenylate kinase activity regulates DNA tethering by Mre11/Rad50 complexes. Bhaskara V, Dupre A, Lengsfeld B, Hopkins BB, Chan A, Lee JH, Zhang X, Gautier J, Zakian V, Paull TT. Mol Cell. 2007 Mar 9;25(5):647-61. doi: 10.1016/j.molcel.2007.01.028. 10.1016/j.molcel.2007.01.028 PubMed 17349953