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pCMV-mAID-BE3
(Plasmid #113420)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113420 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mAID-BE3
  • Alt name
    pCMV-mAICDA-XTEN-D10A-SGGS-UGI-SGGS-NLS
  • Species
    M. musculus (mouse); S. pyogenes and Bacteriophage PBS2
  • Insert Size (bp)
    5040
  • Entrez Gene
    Aicda (a.k.a. Aid, Arp2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH poly reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-mAID-BE3 was a gift from Jia Chen (Addgene plasmid # 113420)
  • For your References section:

    Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L. Nat Biotechnol., 2018 10.1038/nbt.4198