Native promoter dCas9
PurposedCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113656||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2906
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNative promoter and dCas9
- Promoter S. pyogenes Cas9 native promoter
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
Note: This plasmid contains a L64H mutation in Cas9. This mutation is not known to affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Native promoter dCas9 was a gift from Jeffrey Barrick (Addgene plasmid # 113656 ; http://n2t.net/addgene:113656 ; RRID:Addgene_113656)
For your References section:Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure. Geng P, Leonard SP, Mishler DM, Barrick JE. ACS Synth Biol. 2019 Mar 15;8(3):521-531. doi: 10.1021/acssynbio.8b00426. Epub 2019 Feb 15. 10.1021/acssynbio.8b00426 PubMed 30703321