anti-FX(a) Emi HC
PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113665||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA 3.1(+) Hygro
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEmicizumab anti-FX/FXa heavy chain
Specieshumanized monoclonal antibody
- Promoter CMV Promoter
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 Promoter (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:anti-FX(a) Emi HC was a gift from Robert Peters (Addgene plasmid # 113665 ; http://n2t.net/addgene:113665 ; RRID:Addgene_113665)
For your References section:Intrinsic differences between FVIIIa mimetic bispecific antibodies and FVIII prevent assignment of FVIII-equivalence. Leksa NC, Aleman MM, Goodman A, Rabinovich D, Peters R, Salas J. J Thromb Haemost. 2019 Mar 18. doi: 10.1111/jth.14430. 10.1111/jth.14430 PubMed 30887655