PurposeGateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113746||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2540
Vector typeCRISPR ; Gateway Entry Vector
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)726
/ Fusion Protein
- mEGFP (N terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP at your locus of interest (stop codon removed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR-R4R3-mEGFP-N was a gift from Magdalena Bezanilla (Addgene plasmid # 113746 ; http://n2t.net/addgene:113746 ; RRID:Addgene_113746)
For your References section:Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Mallett DR, Chang M, Cheng X, Bezanilla M. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep. 10.1002/pld3.168 PubMed 31523744