AAV-hSyn-Jaws-mRuby2-8X2C
(Plasmid
#114218)
-
PurposeExpresses Jaws-mRuby2 fusion in GABAergic inhibitory neurons in rodent cortex
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 114218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backboneAAV
- Total vector size (bp) 6618
-
Vector typeMammalian Expression, Mouse Targeting, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameJaws (rhodopsin)
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1578
-
MutationNone
- Promoter human synapsin (hSyn)
-
Tag
/ Fusion Protein
- mRuby2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byEd Boyden Lab, MIT
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
AAV-hSyn-Jaws-mRuby2-8X2C was a gift from Xue Han (Addgene plasmid # 114218) -
For your References section:
A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex. Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X. Cell Rep. 2018 Jul 10;24(2):294-303. doi: 10.1016/j.celrep.2018.06.049. 10.1016/j.celrep.2018.06.049 PubMed 29996091