|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114318||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationG12V oncogenic mutation. Constitutively active K-ras protein.
Entrez GeneKRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
- Cloning method Gateway Cloning
Terms and Licenses
Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. Subcloned from Addgene Plasmid #31200. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCLXEBR-pTF-kRasV12 was a gift from Patrick Salmon (Addgene plasmid # 114318 ; http://n2t.net/addgene:114318 ; RRID:Addgene_114318)