Addgene - pHMMa.PH0999 Plasmid Data

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Plasmid 11453: pHMMa.PH0999

Gene/insert name:
pyrazinamidase
Insert size:
540
Species:
P. horikoshii
GenBank ID:
BAA30096 PH0999 NP_142913
Entrez Gene:
PH0999 ()
Vector backbone:
pHMMa
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert:
3188
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
TAATACGACTCACTATAGGG List of Sequencing Primers
3' sequencing primer:
GCTAGTTATTGCTCAGCGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Sung-Hou Kim
Terms and Licenses:
MTA

Comments:

Please note that the plasmid pHMMa.PH0999 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii. Du et al (Biochemistry. 2001 Nov 27. 40(47):14166-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11453" in your Materials and Methods section.