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pGEX-Nedd-4/2522
(Plasmid #11466)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 11466 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-KG
  • Backbone manufacturer
    see PMID 1852137
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nedd4-1
  • Alt name
    Nedd4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    aa 52-777
  • GenBank ID
    U96635
  • Entrez Gene
    Nedd4 (a.k.a. AA959633, AL023035, AU019897, E430025J12Rik, Nedd4-1, Nedd4a, mKIAA0093)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer Amersham 5' pGEX
  • 3′ sequencing primer Amersham 3' pGEX
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-Nedd-4/2522 was a gift from Allan Weissman (Addgene plasmid # 11466)
  • For your References section:

    Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases. Hatakeyama S, Jensen JP, Weissman AM. J Biol Chem. 1997 Jun 13. 272(24):15085-92. 10.1074/jbc.272.24.15085 PubMed 9182527