pRS406 ATG9 stopGFP
(Plasmid
#114669)
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PurposeExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS406
- Backbone size w/o insert (bp) 4297
- Total vector size (bp) 8565
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATG9
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)4265
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MutationChanged the three linker base pairs after the last Arginine to stop codon TAA
- Promoter ATG9
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (unknown if destroyed)
- 3′ cloning site Sac1 (unknown if destroyed)
- 5′ sequencing primer GGT ACT ATT GAC GTC TTG TC
- 3′ sequencing primer AAG AAA TCC GTA AAG ACG CC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was cloned by previous lab members.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS406 ATG9 stopGFP was a gift from Daniel Klionsky (Addgene plasmid # 114669 ; http://n2t.net/addgene:114669 ; RRID:Addgene_114669) -
For your References section:
Phosphorylation of Atg9 regulates movement to the phagophore assembly site and the rate of autophagosome formation. Feng Y, Backues SK, Baba M, Heo JM, Harper JW, Klionsky DJ. Autophagy. 2016;12(4):648-58. doi: 10.1080/15548627.2016.1157237. 10.1080/15548627.2016.1157237 PubMed 27050455