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Addgene

pRS406 ATG9 stopGFP
(Plasmid #114669)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114669 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS406
  • Backbone size w/o insert (bp) 4297
  • Total vector size (bp) 8565
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATG9
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    4265
  • Mutation
    Changed the three linker base pairs after the last Arginine to stop codon TAA
  • Promoter ATG9
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site Sac1 (unknown if destroyed)
  • 5′ sequencing primer GGT ACT ATT GAC GTC TTG TC
  • 3′ sequencing primer AAG AAA TCC GTA AAG ACG CC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was cloned by previous lab members.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS406 ATG9 stopGFP was a gift from Daniel Klionsky (Addgene plasmid # 114669 ; http://n2t.net/addgene:114669 ; RRID:Addgene_114669)
  • For your References section:

    Phosphorylation of Atg9 regulates movement to the phagophore assembly site and the rate of autophagosome formation. Feng Y, Backues SK, Baba M, Heo JM, Harper JW, Klionsky DJ. Autophagy. 2016;12(4):648-58. doi: 10.1080/15548627.2016.1157237. 10.1080/15548627.2016.1157237 PubMed 27050455
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