Addgene - pGALSc104(A503V/A509D) Plasmid Data

Plasmid 1148: pGALSc104(A503V/A509D)

Gene/insert name:
HSP104
Alt name:
5958
Insert size:
2850
Species:
S. cerevisiae (budding yeast)
GenBank ID:
M67479
Entrez Gene:
HSP104 (YLL026W)
Mutation:
A503V/A509D
Vector backbone:
pLA1 (pRS313:GAL1-10)
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5703
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SacI
Site destroyed during cloning:
No
5' sequencing primer:
TACCTCTATACTTTAACGTC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Dominant gain-of-function mutations in Hsp104p reveal crucial roles for the middle region. Schirmer et al (Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1148" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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