Addgene - pLIC.B4.BSU37660 Plasmid Data

Plasmid 11535: pLIC.B4.BSU37660

Gene/insert name:
phosphotransacetylase
Insert size:
969
Species:
B. subtilis
GenBank ID:
NP_391646 BSU37660
Fusion protein or tag:
HisTag-MBP, TEV cleavable
Terminal:
N terminal on backbone
Vector backbone:
pLIC.B4
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7313
Cloning site 5':
SmaI
Site destroyed during cloning:
Yes
Cloning site 3':
SmaI
Site destroyed during cloning:
Yes
5' sequencing primer:
CGTCAGACTGTCGATGAAGCCC List of Sequencing Primers
3' sequencing primer:
GTTGCATCACCTTCACCCTCTCC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Principal Investigator:
Sung-Hou Kim
Terms and Licenses:
MTA

Comments:

Please note that the plasmid pLIC.B4.BSU37660 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate. Xu et al (J Struct Funct Genomics. 2005 Nov 9. ():1-11. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11535" in your Materials and Methods section.