Addgene - RCAS-K-ras Plasmid Data

Gene/insert name:
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Alt name:
K-ras
Alt name:
Ki-ras
Alt name:
Kras
Insert size:
600
Species:
M. musculus (mouse)
GenBank ID:
NC_000072
Entrez Gene:
Kras (RP24-359O2.1, AI929937, K-ras, Ki-ras, Kras-2, Kras2, p21B, ras)
Mutation:
G12D
Vector backbone:
RCAS
(Search Vector Database)
Vector type:
Retroviral
Backbone size w/o insert:
11600
Cloning site 5':
Pme1
Site destroyed during cloning:
Yes
Cloning site 3':
Pme1
Site destroyed during cloning:
Yes
5' sequencing primer:
RCAS-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Sandra Orsulic
Terms and Licenses:
MTA

Comments:

The RCAS vectors that carry oncogenes are harmless for mammalian cells, but can be oncogenic in avian cells since some avian
cells express the receptor for the RCAS virus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of ovarian cancer by defined multiple genetic changes in a mouse model system. Orsulic et al (Cancer Cell. 2002 Feb . 1(1):53-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11549" in your Materials and Methods section.